epitope mapping near the N-terminus of PAPST2 of human origin
recommended for detection of PAPST2 of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with family member PAPST1; also reactive with additional species, including equine, canine, bovine and porcine
PAPST2 Background Information Sulfation is an important post-translational modification of proteoglycans, glycolipids and glycoproteins that requires activity of 3’-phosphoadenosine 5’-phosphosulfate (PAPS), the universal sulfate donor. PAPST2 (Adenosine 3'-phospho 5'-phosphosulfate transporter 2), also known as SLC35B3 (Solute carrier family 35 member B3) is a 401 amino acid Golgi apparatus protein that is predominantly expressed in human colon. Overexpression of either PAPST1 or PAPST2, both of which are members of the nucleotide-sugar transporter family, leads to increased PAPS transport activity within the colon. Knockdown of PAPST2 mRNA results in significantly reduced levels of siacyl 6-sulfo N-acteyllactosamine epitope and overall sulfate incorporation into cellular proteins. There are three isoforms of PAPST2 that are produced as a result of alternative splicing events.
