epitope mapping within a cytoplasmic domain of UBE2J1 of human origin
recommended for detection of UBE2J1 of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with other UBE2 family members; also reactive with additional species, including equine, canine, bovine, porcine and avian
UBE2J1 Background Information Ubiquitination is an important molecular mechanism by which abnormal or short-lived proteins are targeted for degradation by the concerted efforts of at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2J1 (Ubiquitin-conjugating enzyme E2 J1), also known as Ubc6p, CGI-76, NCUBE1, HSPC153 or HSPC205, is a 318 amino acid single-pass type IV membrane protein that belongs to the E2 ubiquitin-conjugating enzyme family and is involved in protein degradation. Localized to the membrane of the endoplasmic reticulum (ER), UBE2J1 catalyzes the attachment of ubiquitin to misfolded membrane proteins, thereby targeting them for proteasomal destruction. This ATP-dependent reaction yields AMP, a diphosphate and a ubiquitin-tagged protein and may be a method of quality control within the ER.
