epitope mapping within an internal region of HCF1 of human origin
recommended for detection of HCF1 of mouse, rat and human origin by WB, IF and ELISA; does not cross-react with HCF1 short form; also reactive with additional species, including equine, canine, bovine and porcine
blocking peptide, sc-13429 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-13429 X, 200 µg/0.1 ml
HCF1 Background Information The herpes simplex virus infection is initiated by VP16, a viral transcription factor that activates the viral immediate-early (IE) genes. VP16 recognizes the IE gene promoters by forming a multiprotein complex with Oct-1 and HCF1 (host cell factor 1), a nuclear protein required for progression through the G1 phase of the cell cycle. This multiprotein complex, called C1, is responsible for transcription of the HSV immediate-early genes and may be critical for the regulation of the HSV lytic-latent cycle. HCF1 is cleaved posttranslationally into separate, but associated, N- and C-terminal polypeptides. The cytoplasmic N-terminal fragment of HCF1 arises by proteolysis of full length HCF1 and associates with VP16. The C-terminal polypeptide of HCF1, distinct from the form of HCF1 that interacts with VP16, exists in a nuclear complex with protein phosphatase 1.
