shRNA Plasmid 는 일반적으로 유전자 발현을 knockdown 시키도록 만들어진 목표 특정 19-25 nt (plus hairpin) shRNA 를 암호화하는 3 개 - 5 개의 lentiviral vector plasmid 들로 이루어진 pool 로 조성되어 있습니다.
shRNA sequences correspond to UBE2F siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particle 은 유전자 발현억제를 위한 transduction-ready viral particles 로 제공됩니다.
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to UBE2F siRNA siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. 상세정보는 여기를 클릭하세요.
