santa cruz biotechnology, inc.
mouse monoclonal IgG2b , 200µg/ml raised against amino acids 1-274 representing full length γ-GCSm of human origin recommended for detection of γ-GCSm of mouse, rat and human origin by WB, IP, IF and ELISA
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Species
유전자 이름 (Gene Name)
유전자 ID
염색체 위치
Isoform (mRNA) Accession #
Protein Accession #
OMIM™ Number
Human
GCLM
2730
1p22.1
P48507
601176
Mouse
Gclm
14630
3 G1
O09172
없음
γ-GCSm Background Information Gamma-glutamylcysteine synthetase (©-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. ©-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human ©-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of ©-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
γ-GCSm (F-8)
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γ-GCSm (F-8): sc-166603. Western blot analysis of γ-GCSm expression in K-562 (A), A549 (B) and c4 (C) whole cell lysates and rat liver (D) and mouse liver (E) tissue extracts.
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