Pbs2 Background Information Yeast cells regulate their internal osmolarity in response to the environment via a MAP kinase cascade (1). Map kinase cascades comprise an essential branch of signal transduction, transmitting extracellular signals to the cytoplasm or nucleus. The core of these cascades consist of a MAP kinase (Mitogen Activated Protein Kinase, also called ERK, for Extracellular-Regulated protein Kinase) as well as one or more up-stream regulatory kinases (MAPKKs or MEKs, for MAP/ERK Kinase) (2,3). High external osmolarity leads to the activation of the MAPKK Pbs2, which activates the MAP kinase Hog1 (4,5). Sho1 also activates Hog1 in response to oxidative stress, and Hog1 (also called Ssk3) is thought to activate a transcription factor that up-regulates the production of osmo-regulatory proteins (6,7). Kex2 is a protease that regulates metabolism by mediating insulin processing (8).
Pbs2 (y-118)
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Pbs2 (y-118): sc-28650. Western blot analysis of yeast recombinant Pbs2 fusion protein.
