epitope mapping near the N-terminus of DHS of human origin
recommended for detection of DHS of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
DHS Background Information Deoxyhypusine synthase (DHS) is crucial for the post-translational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A). Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH). eIF-5A is essential for eukaryotic cell proliferation. Deoxyhypusine synthase, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.
DHS (N-15)
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DHS (N-15): sc-46917. Western blot analysis of DHS expression in non-transfected: sc-117752 (A) and human DHS transfected: sc-177128 (B) 293T whole cell lysates.
