epitope mapping within an internal region of β-1,3-Gal-T4 of human origin
recommended for detection of β-1,3-Gal-T4 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and porcine
β-1,3-Gal-T4 Background Information ∫-1,3-Gal-T4 (∫-3-galactosyltransferase 4), also known as Gal-T2, is a member of the ∫-1,3-Gal-T family of type II membrane bound glycoproteins. It is expressed in various tissues including pancreas, heart, skeletal muscle and, to a lesser extent, brain, kidney, placenta, lung, liver and some cancer cells. ∫-1,3-Gal-T4 localizes to the Golgi apparatus. It consists of an N-terminal cytoplasmic domain, a transmembrane domain, a hydrophobic stem region and a catalytic domain. Unlike other ∫-1,3-Gal-T family members, ∫-1,3-Gal-T4 has only one N-linked glycosylation site. Enzymatic activity of ∫-1,3-Gal-T4 is dependent on glycosylation at this site. Active ∫-1,3-Gal-T4 catalyzes the addition of galactose (from the sugar donor UDP-Gal) to the N-acetylgalactosamine residue of gangliosides GM2, GD2 and GT2 or to the glycolipid GA2.
