shRNA Plasmid 는 일반적으로 유전자 발현을 knockdown 시키도록 만들어진 목표 특정 19-25 nt (plus hairpin) shRNA 를 암호화하는 3 개 - 5 개의 lentiviral vector plasmid 들로 이루어진 pool 로 조성되어 있습니다.
shRNA sequences correspond to Rab 22A siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particle 은 유전자 발현억제를 위한 transduction-ready viral particles 로 제공됩니다.
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. 상세정보는 여기를 클릭하세요.
